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1.
Fisioter. Bras ; 21(2): 204-210, Mai 16, 2020.
Article in Portuguese | LILACS | ID: biblio-1282972

ABSTRACT

Introdução: As associações de Terapias Combinadas de Ultrassom e Eletroterapia (TCUE) no mercado nacional podem ser utilizadas através de diferentes equipamentos. Na prática clínica tem-se relatado que o uso das TCUE tem eficácia no tratamento da adiposidade localizada. Objetivo: Avaliar os efeitos da terapia combinada (TCUE) no tratamento da adiposidade localizada. Métodos: Trata-se de um estudo experimental, composto por uma amostra de 15 coelhos, de ambos os sexos, da espécie Oryctolagus Cuniculus, divididos aleatoriamente em três grupos: G1- grupo TCUE-M que utilizou efeitos do ultrassom com aplicação de correntes estereodinâmicas (n = 5); G2- grupo TCUE -H que utilizou uma combinação de ultrassom e corrente Aussie (n = 5) e G3- controle (n = 5) nenhuma terapia. Resultados: As análises dos resultados revelaram pequenas alterações na camada adiposa na maioria dos coelhos integrantes dos grupos tratados. Conclusão: A análise das lâminas histológicas apresentou irregularidades na membrana celular adiposa mostrando diferentes tamanhos de células. (AU)


Introduction: Associations of Combined Ultrasound Therapy and Electrotherapy (TCUE) can be used in the domestic market through different equipment. Some authors report that the use of TCUE has efficacy in the treatment of localized adiposity. Objective: To evaluate the effects of combined therapy (TCUE) in the treatment of localized adiposity. Methods: This is an experimental study consisting of a sample of 15 rabbits of both sexes of the species Oryctolagus Cuniculus, randomly divided into three groups: G1- TCUE-M group that used ultrasound effects with the application of stereodynamic currents (n = 5); G2-TCUE-H group that used a combination of ultrasound and current Aussie (n = 5) and G3-control (n = 5) no therapy. Results: Analyzes of the results revealed small alterations in the adipose layer in most of the rabbits in the treated groups. Conclusion: Analysis of the histological slides presented irregularities in the adipose cell membrane showing different cell sizes. (AU)


Subject(s)
Humans , Combined Modality Therapy , Adiposity , Ultrasonic Therapy , Adipose Tissue , Electric Stimulation Therapy
2.
Rev. colomb. ciencias quim. farm ; 49(1): 70-88, Jan.-Apr. 2020. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1144339

ABSTRACT

SUMMARY This study aimed at evaluating effective methods for breaking the hard and insoluble spores of Ganoderma lucidum to recover functional biomolecules. Rupture techniques were evaluated such as manual maceration (RM), maceration with spheres of various materials (BR), and microwave exposure plus maceration with steel/ chrome spheres (MBR1). Spore rupture was evaluated using UV-Vis spectroscopy, which showed vibrations of 2955, 1642, 1240, 1080 and 1746 cm-1 corresponding to changes in spore walls. The MBR1 extract contained the largest amounts of carbohydrates (19.80 mg.g-1 spores) and polyphenols (2.21 mg.g-1 spores), whereas the BR extract had higher antioxidant activity (57.22%Inb DPPH). The MBR1 and BR extracts contained 62.2 and 73.5% glucose, respectively. Both methods also involved significant extraction of carbohydrates and proteins. The best way to extract biomolecules from spore walls is to perform a microwave heat treatment and break the walls with steel/chrome spheres; this produces large quantities of carbohydrates with antioxidant properties.


RESUMEN El objetivo de este estudio fue evaluar varios métodos de ruptura de las esporas de Ganoderma lucidum y extraer sus propiedades bioactivas. Para este propósito se evaluaron diferentes técnicas de rompimiento como: la maceración manual (RM), la maceración con esferas de diversos materiales (BR) y la exposición a microondas junto la maceración de las esporas con esferas de acero/cromo (MBR1). La ruptura de las esporas fue evaluada por espectroscopia UV-Vis, la cual mostró que las vibraciones 2955, 1642, 1240, 1080 y 1746 cm-1 correspondieron a cambios estructurales en las paredes de las esporas. El extracto MBR1 presento el mayor contenido de carbohidratos (19,80 mg.g-1) y polifenoles (2,21 mg.g-1), mientras que el extracto BR tuvo una mayor actividad antioxidante (57,22% Inb DPPH). Los extractos MBR1 y BR también presentaron en el análisis de monosacáridos un 62,2 y 73,5% de contenido glucosa. Como conclusión la mejor metodología para extraer biomoléculas de las paredes de las esporas de G. lucidum fueron el tratamiento térmico con microondas y la ruptura de las paredes con esferas de acero/cromo, porque este proceso permitió la extracción de una mayor cantidad de carbohidratos con posibles propiedades antioxidantes.

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